Technology: CRISPR expression profiles for unlocking the Hidden Kinome

• Michael T. McManus, Ph.D.
  Professor, University of California, San Francisco
  Vincent and Stella Coates Endowed Chair
  Director, UCSF Keck Center for Noncoding RNAs
  Core Director, UCSF Sandler Lentiviral RNAi Core
  Department of Microbiology and Immunology
  UCSF Diabetes Center
  
  

Overview

The McManus project uses Cas9-based technologies (CRISPRi, CRISPRa, and wtCRISPR) to interrogate GPCR, NR, ion channel, and kinase genes whose perturbation can cooperate or interfere with signal transduction, specifically cell proliferation, cell death, and differentiation. Dr. Michael T. McManus’ work for IDG is applying the CRISPR-CAS technology to unlock the hidden kinome (and will expand to other IDG protein classes) allowing for measuring the connection between genes and pathways by the utility of gene expression, gene activation or gene edition. Goals are to validate these Tdark matter and additional screens for epistatis.